The role of Hedgehog protein and gene methylation in the pathogenesis of esophageal cancer / 中国基层医药
Chinese Journal of Primary Medicine and Pharmacy
; (12): 2506-2509,后插7, 2017.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-617879
Biblioteca responsável:
WPRO
ABSTRACT
Objective To explore the role of the Hedgehog singaling in the pathogenesis of esophageal cancer.Methods Tissue samples of normal esophageal mucosa,low-grade dysplasia,high-grade dysplasia and esophageal cancer were collected.Immunohistochemical stain,Western-blot and RT-PCR were employed to detect theexpression of Hedgehog protein and gene,methylation special PCR was used to detect the methylation status of Hedgehog gene.Results There was no difference in the expression of SHh protein among nornaml esophageal and low-grade dysplasia,high-grade dysplasia and esophageal cancer(LGD vs.Nt=1.96,P=0.67;HGD vs.ECt=1.59,P=0.53).However,the expression of SHh protein in high-grade dysplasia and esophageal cancer was higher than that in normal esophageal mucosa and low-grade dysplasia(HGD vs.Nt=0.593,P=0.004;HGD vs.LGDt=1.308,P=0.325;EC vs.Nt=0.292,P=0.000;EC vs.LGDt=0.734,P=0.004).The expression of Hedgehog gene in esophageal cancer was higher than that in normal esophageal mucosa,low-grade dysplasia and high-grade dysplasia(EC vs.Nt=0.909,P=0.019,EC vs.LGDt=0.398,P=0.007;EC vs.HGDt=0.843,P=0.012).The gene methylation in esophageal cancer was lower than that in normal esophageal mucosa,low-grade dysplasia and high-grade dysplasia(EC vs.Nt=0.0340,P=0.000;EC vs.LGDt=0.102,P=0.000;EC vs.HGDt=0.367,P=0.018).Conclusion Hedgehog signaling may play a role in the pathogenesis and development of esophageal cancer,however,demethylation of Hedgehog gene may be one of the mechanism that cause the activity of oncogene in esophageal cancer.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo de etiologia
Idioma:
Chinês
Revista:
Chinese Journal of Primary Medicine and Pharmacy
Ano de publicação:
2017
Tipo de documento:
Artigo