Neuroprotective Effects of Betaxolol Mediated by Heme Oxygenase-1 Induction in RGC-5
Journal of the Korean Ophthalmological Society
; : 113-119, 2016.
Article
em Ko
| WPRIM
| ID: wpr-62060
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: To evaluate the neuroprotective effects of betaxolol (betaxolol hydrochloride) under hypoxic conditions using retinal ganglion cells (RGC-5) and determine whether heme oxygenase-1 (HO-1) expression exerts cytoprotective effects. METHODS: In this study, cultured RGC-5 cells were incubated with different concentrations of betaxolol hydrochloride (0.1 microM, 1 microM or 5 microM) and with 10 microM zinc protoporphyrin (ZnPP), in a hypoxia incubator (1% O2, 5% CO2, 94% N2) for 48 hours and the cell viability of each group was determined. Additionally, cell viability was measured after RGC-5 cells were incubated with 5 microM of brinzolamide (Azopt(R)), brimonidine tartrate (Alphagan(R)) or travoprost (Travatan(R)). RGC-5 cells were divided into three groups and incubated under three different conditions, normoxia group (20% O2, 5% CO2), hypoxia group (1% O2, 5% CO2) and the group with 5 microM of Betoptic S(R) treated under hypoxic conditions (hypoxia, Betoptic S(R)). After incubation for 4, 8, 12 and 24 hours, HO-1 expression was analyzed using Western blotting. RESULTS: Cell viability significantly increased in RGC-5 cells treated with Betoptic S(R) compared with other antiglaucoma agents. Increased levels of HO-1 expression indicate its relevance in cell viability. Furthermore, increased RGC-5 cell viability by Betoptic S(R) was significantly reduced in the HO-1 inhibitor ZnPP-treated group. CONCLUSIONS: We reaffirmed the known cytoprotective effects of Betoptic S(R) and the results suggests that HO-1 expression exerts cytoprotective effects against hypoxia.
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Base de dados:
WPRIM
Assunto principal:
Células Ganglionares da Retina
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Zinco
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Betaxolol
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Sobrevivência Celular
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Western Blotting
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Fármacos Neuroprotetores
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Heme Oxigenase-1
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Tartarato de Brimonidina
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Travoprost
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Heme
Idioma:
Ko
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2016
Tipo de documento:
Article