Increased IL-12 , but Depressed IL-18 Production after In Vitro Stimulation with a 30-kDa Mycobacterial Antigen in Tuberculous Pleural Mononuclear Cells
Journal of Bacteriology and Virology
; : 239-248, 2001.
Article
em En
| WPRIM
| ID: wpr-64249
Biblioteca responsável:
WPRO
ABSTRACT
In this study, we investigated interleukin (IL)-18 and IL-12 following in vitro stimulation with either the 30-kDa or purified protein derivative (PPD) antigens (Ag) of pleural mononuclear cells from 12 cases of tubercular pleurisy (TB-PMC) and 8 cases of malignant pleurisy (MG-PMC). Ag-stimulated TB-PMC produced significantly more IL-12 than did MG-PMC and the levels correlated with those of IFN - gamma. Although elevated IL-18 levels were found in freshly isolated pleural fluids, in vitro IL-18 production in response to either Ag was dramatically decreased in TB-PMC. Pro-IL-18 mRNA was detected before and after Ag stimulation in TB patients. Supernatants from the Ag-stimulated TB-PMC significantly suppressed IL-18 production in normal peripheral blood mononuclear cells (PBMC) and primary malignant cells over an 18 h incubation period. In addition, this suppressive activity was not inactivated by either heat or trypsin. Our findings imply that modulation of IL-12 and IL-18 levels may contribute to the Th1 elevation induced in human TB-P VIC by the 30-kDa and PPD antigens.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Pleurisia
/
Tuberculose Pleural
/
RNA Mensageiro
/
Tripsina
/
Interleucinas
/
Interleucina-12
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Interleucina-18
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Temperatura Alta
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Mycobacterium tuberculosis
Limite:
Humans
Idioma:
En
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2001
Tipo de documento:
Article