Cloning,weukaryotic expremion of the gene encoding glyceraidehydes-3-phosphate dehydrogenase fromperiodic Brugia malayi / 中国地方病学杂志
Chinese Journal of Endemiology
; (6): 609-612, 2008.
Article
em Zh
| WPRIM
| ID: wpr-643288
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WPRO
ABSTRACT
Objective To clone and express the encoding sequence of glyceraldehydes-3-phosphate dehydrogenase(GAPDH)from periodic Brugia molayi(Bm).Methods Total RNA was extraeted from periodic Brugic malayi.The BmGAPDH gene was amplified by RT-PCR.The PCR product was cloned and then subeloned into pcDNA3.1(+)vector.The recombinant plasmids were screened and identified by digestion with restriction enzyme and PCR amplification,and were transformed into COS-7 cell subsequently.The expressed protein was identified by SDS-PAGE.Results BmGAPDH mRNA was highiy expressed in transfected COS-7 cell.The deduced amino acid sequence was identical with that of BmGAPDH.The recombinant pnotein wag about Nr 43 000.Conclusion The recombinant plasmid peDNA3.1(+)-BmGAPDH has been constructed and the protein has been expressed correctly.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Endemiology
Ano de publicação:
2008
Tipo de documento:
Article