Analysis of Chromosomal Aberrations in Thyroid Papillary Carcinomas Using Comparative Genomic Hybridization (CGH) / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
; : 1369-1376, 2005.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-645298
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND AND OBJECTIVES:
Cancer of the thyroid is the sixth common cancer in Korea, and fourth common among the Korean women, in particular. Aming the prevalent carcinomas of thyroid, the papillary thyroid carcinoma is the most frequent type. Genomic instability is the characteristic of nearly all tumors as well as thyroid cancers. However, despite the high frequency of papillary thyroid carcinomas, their chromosomal alterations are poorly characterized in Korea. Comparative genomic hybridization (CGH) is a new fluorescence in situ hybridization (FISH) technique to identify genomic imbalances in cancers. In this study, CGH was carried out with the aim of analyzing non-random chromosomal aberrations involved in papillary thyroid carcinomas. MATERIALS ANDMETHOD:
CGH was carried out. Biotin-labeled tumor DNA and digoxigenin-labeled normal DNA were co-hybridized to normal metaphase cells. Then, the ratio of fluorescence was analyzed by an image analyzer. In array-CGH, Cy3 labeled tumor DNA and Cy5 labeled normal DNA were hybridized to microarray template, and then image analysis was performed by microarray image analyzer.RESULTS:
Gains of 22q13, 6p24, 7p13, 7q21, 7q31, 8q24, 17q24 and 19p13.3 were found frequently.CONCLUSION:
Non-random aberrations which were disclosed in this study might be candidate regions for the abnormal genes involved in papillary thyroid cancer.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Glândula Tireoide
/
DNA
/
Neoplasias da Glândula Tireoide
/
Carcinoma Papilar
/
Aberrações Cromossômicas
/
Hibridização In Situ
/
Instabilidade Genômica
/
Hibridização Genômica Comparativa
/
Fluorescência
/
Hibridização Genética
Tipo de estudo:
Estudo prognóstico
Limite:
Feminino
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Coreano
Revista:
Korean Journal of Otolaryngology - Head and Neck Surgery
Ano de publicação:
2005
Tipo de documento:
Artigo