Osteoinduction Using Recombinant Bone Morphogenetic Protein-7 Gene / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
; : 598-606, 2004.
Article
em Ko
| WPRIM
| ID: wpr-645802
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: The aim of this study was to develop recombinant human Bone Morphogenetic Protein-7 (BMP7)-stable cells and recombinant human BMP7 adenoviruses (AdBMP7) for osteoinduction and osteoregeneration in musculoskeletal diseases. MATERIALS AND METHODS: The human BMP7 cDNA was amplified from a human osteosarcoma cell line, U2OS using a reverse transcription-polymerase chain reaction and cloned into a eukaryotic expression vector. The BMP7-stable HEK293 cells (HEK293/BMP7) were prepared by transfecting the recombinant BMP7 plasmid vector. The recombinant human BMP7 adenovirus (AdBMP7) was constructed using the AdEasy vector system. The BMP7 expression levels in HEK293/BMP7 and AdBMP7 were measured by activity staining for alkaline phosphatase in mouse C2C12 promyoblast cells. The BMP7-stable HEK293 cells, AdBMP7 itself, or AdBMP7-transduced human fibroblasts were injected into the subcutaneous tissues and the calf muscles of immunocompromised mice. The amount of ectopic bone formation was evaluated by radiographic and histological analyses. RESULTS: Ectopic bone formation was observed after injecting the BMP7-stable HEK293 cells with either the AdBMP7 itself or AdBMP7-transduced human fibroblasts into the subcutaneous tissues and calf muscles of immunocompromised mice. CONCLUSION: These results showed that HEK293/BMP7 cells and AdBMP7 have a significant potential for bone formation and the regeneration of various bone diseases.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Osteogênese
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Plasmídeos
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Regeneração
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Doenças Ósseas
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Terapia Genética
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Osteossarcoma
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Linhagem Celular
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Adenoviridae
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Células Clonais
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Doenças Musculoesqueléticas
Limite:
Animals
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Humans
Idioma:
Ko
Revista:
The Journal of the Korean Orthopaedic Association
Ano de publicação:
2004
Tipo de documento:
Article