The Effect of Human Amniotic Membrane, Epidermal Cells and Marrow Mesenchymal Stem Cells in Healing a Skin Defect / 대한정형외과학회잡지

ABSTRACT

PURPOSE: The purpose of this study was to investigate the transplantation results of human amniotic membrane (HAM), epidermal cells, or marrow mesenchymal stem cells (MSCs) in healing a skin defect. MATERIALS AND METHODS: Defects (full-thickness) in rabbits were treated with HAM alone (group A), HAM injected with cultivated epidermal cells (group B), HAM injected with cultivated MSCs (group C), or Vaseline gauze (group D). Tissue granulation, regeneration, re-epithelization and healing time were measured. Defects and healed area were calculated 2 weeks after surgery. RESULTS: The mean healing area was 67.5%, 81.7%, 83.2% and 49.5% in each group, with all treatment groups significantly different than group D (p<0.01), and groups B and C compared higher than group A (p<0.05). The healing time of groups A, B, and C was 5.7 to 6.4 days faster than that of group D (p<0.01). Histologic analysis showed that the new epidermis covered nearly the whole wound surface in group B and C, and contained granulated tissue with fibroblasts, capillaries, and collagen. CONCLUSION: HAM grafts injected with cultivated epidermal cells or MSCs promoted healing of skin defects.