Effect of Intermittent Administration of Parathyroid Hormone on Fracture Healing in Ovariectomized Rats / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
; : 207-217, 1999.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-650649
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
This study was designed to compare fracture healing in normal and ovariectomized rat, and to evaluate the effect of intermittent administration of parathyroid hormone on fracture healing in osteopenic animal model, MATERIALS ANDMETHODS:
Four-months-old mature female Sprague-Dawley rats were randomly elivided into 5 groups. Group I underwent a sham operation, and others (Group II-V) were ovariectomized. At three months after ovariectomy or sham operation, standardized bilateral transverse tibial fractures were created and intramedullary nailings with Kirschner wire were performed. The rats were then treated with daily subcutaneous injection of placebo in Groups I and II, 17beta-estradiol in Group III, low doses of recombinant human PTH (1-84) in Group IV, and high doses of recombinant human PTH (1-84) in Group V for 4 weeks. At day 30 of post-fracture the animals were sacrificed and fracture healing was assessed with histologic/histomorphometric analysis and three-point bending mechanical testing.RESULTS:
On histologic/histomorphometric evaluation of sham operation group, the fracture callus mainly consisted of dense trabecular bone. On the other hand, Groups II and III seemed to have much looser cancellous network, abundant in fibrous marrow. In parathyroid hormone-treated g roups, external callus consisted of more dense trabecular, woven bone than that of Groups II or III, and especially the high doses of parathyroid hormone-treated group was comparable to the sham operation group in terms of per cent trabecular bone volume (Group I>V>IV>III=II, PIV>III=II, PIV=III=II, PIV=II=III, P<0.05).CONCLUSIONS:
On the basis of this study, it may be concluded that fracture healing is delayed in the ovariectomy-induced osteopenic rat model. Our experiment also showed dose-related stimulation of parathyroid hormone in the strength of fracture, and that antiresorptive agents such as estrogen had no effect. Further study is needed in large animal model, and attention should be focused on systemic/long-term effect of parathyroid hormone and its relationship with local growth factors in fracture healing.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Hormônio Paratireóideo
/
Fraturas da Tíbia
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Medula Óssea
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Calo Ósseo
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Ovariectomia
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Ratos Sprague-Dawley
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Consolidação da Fratura
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Modelos Animais
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Estrogênios
Tipo de estudo:
Ensaio clínico controlado
/
Estudo prognóstico
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Coreano
Revista:
The Journal of the Korean Orthopaedic Association
Ano de publicação:
1999
Tipo de documento:
Artigo