Characteristics of protein from red crab (Chionoecetes japonicus) shell by commercial proteases
The Korean Journal of Nutrition
; : 429-436, 2012.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-651206
Biblioteca responsável:
WPRO
ABSTRACT
This study was performed to examine the characteristics of protein of red crab (Chionoecetes japonicus) shell powder hydrolyzed by commercial proteases. Red crab shell was digested by commercial proteases, such as Protamex (P), Neutrase (N), Flavourzyme (F), Alcalase (A), Protease M (PM) and Protease A (PA). Protein yield analyzed by Biuret assay, absorbance at 280 nm and brix revealed that PA was the enzyme having the highest proteolytic activity. SDS PAGE showed that molecular weight of proteins produced by protease treatments was various and below 150 kDa. Combinational treatment of proteases (PA + P, PA + PM, PA + F, PA + A) was tried whether these increase protein hydrolysis from red crab shell powder compared to a PA single treatment. Soluble protein content was similar, but amino acid concentration by combinational treatments was higher than PA single treatment [PA + P 247.4 mg/g > PA + F (206.4 mg/g) > PA + A (133.4 mg/g) > PA + PM (59.1 mg/g) > PA (54.9 mg/g)]. Amino acid composition by combinational treatments was slightly different. Most abundant essential amino acids were phenylalanine, glycine, alanine, and leucine, whereas tyrosine and cystine were not detected.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peptídeo Hidrolases
/
Endopeptidases
/
Fenilalanina
/
Tirosina
/
Biureto
/
Metaloendopeptidases
/
Subtilisinas
/
Proteínas
/
Cistina
/
Alanina
Idioma:
Coreano
Revista:
The Korean Journal of Nutrition
Ano de publicação:
2012
Tipo de documento:
Artigo