Tamibarotene alleviates cerebral ischemia reperfusion injury in rats / 基础医学与临床

ABSTRACT

Objective To investigate the effect of tamibarotene(Am80)on cerebral ischemia reperfusion injury. Methods Rats were randomly divided into sham operation group(Sham),model group(I/R) and tamibarotene in-tervention group (Am80, intragastric administration of Am80 6 mg/kg). The middle cerebral artery occlusion (MCAO) was established by suture method. At 24 h after reperfusion,the brain was removed. And the neurological behavioral score was assessed by double blind method.The cerebral infarction volume was measured by TTC staining. The proteins of RARα,Bcl-2,Bax and mRNA were examined by Western blot and RT-qPCR,respectively. Results Am80 significantly improved the neurological deficits of MCAO rats and effectively reduced the infarct volume. And upregulated the level of RARα and Bcl-2, decreased the expression of Bax. Conclusions Am80 has a protective effect on cerebral ischemia-reperfusion in rats,and its effect may be related to anti-apoptosis.