Production and application of monoclonal antibody against major histocompati-bility complex class Ⅰrelated chain A and B / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 1514-1519, 2017.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-660052
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
Generation and identification of hybridoma to produce monoclonal antibody against MICA/B.Methods:
SP2/0 cells were fused with murine splenocyte immunized with recombinant protein rMICA?012 to get hybridoma cell lines. The titer of the monoclonal antibody produced by 9B10 cell line was determined by ELISA and its specificity was tested by Western blot,Luminex mutiplex microsphere-based immunoassay and immunofluorescence assay.Results:
Six hybridoma cell lines were selected by ELISA screening test. The minimum reaction concentration of mAb 9B10 was 0. 02 ng/μl,and the specificity of mAb 9B10 was determinated by Western blot,Luminex mutiplex microsphere-based immunoassay and immunofluorescence.Conclusion:
The monoclonal antibody 9B10 was available to apply for the detection of MICA and MICB expression.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2017
Tipo de documento:
Artigo