Simultaneous determination of seven constituents in Artemisia argyi leaves by HPLC / 中成药

ABSTRACT

AIM To establish an HPLC method for the simultaneous content determination of neochlorogenic acid,cryptochlorogenic acid,chlorogenic acid,caffeic acid,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C in the leaves of Artemisia argyi Levl.et Vant..METHODS The analysis of 50％ methanol extract of A.argyi leaves was performed on a 30 ℃ Prevail C1s column (4.6 mm ×250 mm,5 μmn),with the mobile phase comprising of acetonitrile-water flowing at 1.0 rnL/min in a gradient elution manner,and the detection wavelength was set at 325 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥ 0.999 5),whose average recoveries were 98.28％-101.11％ with the RSDs of 1.04％-2.59％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of A.argyi leaves.