Effects of low-dose low molecular weight heparin on lipopolysaccharide-induced acute lung injury / 重庆医学

ABSTRACT

Objective To explore the effect of low-dose low molecular weight heparin(LMWH) on acute lung injury(ALI) induced by lipopolysaccharide(LPS).Methods Thirty-six male SD rats were divided into 3 groupsnormal control group(A),LPS group(B) and LPS plus LMWH group(C),12 cases in each group.ALI was induced by intraperitoneal injection of LPS 6 mg/kg in group B,C.The group C accepted intraperitoneal injection of LMWH 100 U/kg,the groups B accepted intraperitoneal injection of the same volume of normal saline.The animals were killed after 6 h,the pathological changes of the lung were observed under light microscope.Arterial blood gases,lung wet to dry ratio (W/D) and protein content in BALF were detected;the levels of MPO and MDA in lung tissue were determined.The levels of serum interleukin1β(IL-1β),IL-6 and tumor necrosis factor-α(TNF-α) were determined by ELISA method.Results The PaO2 and pH values in the group B and C were significantly lower than those in the group A,and which in the group C were markedly increased compared with those in the group B(P＜0.05);the lung W/D ratio,protein content in BALF,MDA and MPO levels in the lung tissue in the group B and C were significantly higher than those in the group A(P＜0.01),while the lung W/D ratio,protein content in BALF,MDA and MPO levels in the lung tissue in the group C were significantly decreased compared with the group B (P＜0.05).The levels of plasma TNF-α,IL-1β and IL-6 levels in the group B and C were markedly increased compared with the group A,while which in the group C were significantly decreased compared with those in the group B (P＜0.01).Conclusion The LMWH treatment might attenuate LPS-induced acute lung injury.