Effect of Marine Capsules on BALB/c Human Hepatocellular Carcinoma Cell Line SMMC-7721 in vitro / 中国药师

ABSTRACT

Objective:To observe the effect of marine capsules on BALB/c human hepatocellular carcinoma cell line SMMC-7721 in vitro. Methods:Human hepatoma SMMC-7721 cell line was cultured in vitro to establish a hepatoma tumor model, and was random-ly divided into the model control group, 5-Fu group (25 mg·kg-1), marine capsules high dose group (90 mg·kg-1), marine cap-sules low dose group (45 mg·kg-1 ) and the blank control group with 10 mice in each and 14-day continuous administration. Blood was taken from the eyeball and serum was isolated. The tumor strains were picked, the wet weight was obtained, the inhibition rate was calculated, and the pathological changes of tumor tissues were observed after HE staining. Results: Compared with that in the model control group, the serum IL-2 content significantly increased in the marine capsules high and the low dose groups ( P<0. 01, P<0. 05). The in vitro growth of tumor (P<0. 001, P<0. 05) was inhibited with the inhibition rate of 41. 56% and 27. 78% respectively for the high dose group and the low dose group, and the effects of the high dose group had no significant differences with those of 5-Fu group (P>0. 05). The nuclear staining of tumor cells was lighter and the number of tumor cells decreased in the two marine capsules groups. Conclusion:Marine capsules have certain inhibitory effects on BALB/c human hepatocellular carcinoma cell line SMMC-7721 in vitro.