Observation of Whether to Mix Evenly the Serum Samples Preserved at-20℃ for Clinical Routine Biochemical Test Results / 现代检验医学杂志

ABSTRACT

Objective To explore the change of conventional biochemical test results of serum specimens saved-20℃ and blending of its effects.Methods The fresh serum of 24 patients were conducted routine biochemical tests,respectively in four groups of numbers in the same capacity of 1 ml of the sample tube,a set of 25℃ kept 24 hours a day,a group of 4 ℃ refrigerated for 24 hours,24 hours at-20℃ save the two groups (a group was not incorporated,a set of blended),and the four groups of serum samples in biochemical results comparing with the initial results.Results 25℃ at room temperature preservation after 24 hours,ALT,AST,TBIL and DBIL biochemical indicators of four average were below the initial results (t=2.130,2.308,2.130 and 2.308,P=0.042,0.029,0.042 and 0.003),there were no statistically significant difference between the rest of the biochemical index results comparison (P＞ 0.05).4℃ refrigerator after 24 hours,TBIL,DBIL levels below the initial level (t=2.103 and 2.089,P=0.045 and 0.046),and the rest of the biochemical indicators to compare differences had no statistical significance (P＞0.05).-20℃ refrigerated in the two groups of samples,not each sample of serum biochemical results compared with the initial results,were kept statistically significant difference (t=-4.218～11.710,all P＜0.05),while serum samples of 15 items and biochemical indexes of blending the results compared with the initial results,the average level of had no statistical significance (P＞0.05).Conclusion Serum samples of the chemical composition of the homogeneity in the 4 ℃ refrigerator will not change,but will not be able to timely detection of serum samples in the preservation,and minus-20 ℃ refrigerator on the serum samples of a significant difference was found in the lower concentration of chemical components,compete on low,and tests conducted before fully blending can ensure the precision of its biochemical test results.