Effects on phenotype and function of mouse CIK cells treated with carboxylated single-walled carbon nanotubes / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effect on the phenotype and function of CIK cells induced from splenic lymphocyte in mice treated with carboxylated single-walled carbon nanotubes.Methods:CIK cells were treated with carboxylated single-walled carbon nanotubes at different concentrations.Cell growth status was observed using inverted fluorescence microscopy.The killing effect was de-termined by MTS method.Immunophenotype was analyzed by flow cytometry.Results: With the increase of the effect target ratio,the killer rate of CIK was gradually enhanced and the optimal effect target ratio was 201.With the increase of the dose of carboxylated single-walled carbon nanotubes,the proportion of Treg cells decreased.When CIK cells were treated with 0.5 μg/ml carboxylated single-walled carbon nanotubes(CNTs),the proportion of CD3+CD4+and CD3+CD8+was significantly higher than that of the control group(P<0.05),and the killing effects of CIK anchieved best results to B16 cells,H22 cells and RM-1cells.Conclusion: The carboxylated single-walled carbon nanotubes enhanced the ability of CIK cells to kill tumor cells,significantly which provide has potential value in tumor drug development.