Determination of Chlorogenic Acid in Honeysuckle Stem and Honeysuckle from Different Sources in Differ-ent Harvest Periods by HPLC / 中国药师
China Pharmacist
; (12): 682-684, 2015.
Article
em Zh
| WPRIM
| ID: wpr-669957
Biblioteca responsável:
WPRO
ABSTRACT
Objective:To establish an HPLC method for the determination of chlorogenic acid in honeysuckle stem and honeysuck-le from different sources in different harvest periods. Methods:A Waters C18 column(250 mm × 4. 6 mm,5 μm)was used. The mo-bile phase consisted of acetonitrile-0. 4% H3 PO4(10:90)with the flow rate of 1. 0 ml·min-1 . The detection wavelength was 327 nm and the column temperature was 30℃. An external standard method was established for the determination of chlorogenic acid in honey-suckle stem and honeysuckle in six different harvest periods from three sources. Results:There was a good linear relationship within the range of 0. 065-1. 300 μg for chlorogenic acid(r=0. 999 8). The content of chlorogenic acid in honeysuckle was the highest in Sep-tember and October. The content of chlorogenic acid in Lonicera acuminata was the highest among the honeysuckle stem from three dif-ferent sources. Conclusion:The content of chlorogenic acid in honeysuckle has a certain relationship with the harvest time,which can provide theoretical basis for the choice of harvest time for honeysuckle stem.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
China Pharmacist
Ano de publicação:
2015
Tipo de documento:
Article