Effect of Qingdu Granules on Breast Cancer in Rats via Suppressing Immune Escape / 中国药师

ABSTRACT

To study the effect and mechanism of Qingdu granules on the tumor growth of 7, 12-dimethyl-benz[ a] an-thracene ( DMBA)-induced breast cancer in Sprague-Dawley rats. Methods: DMBA was used to induce breast cancer in rats. The tumor inhibition of Qingdu granules was observed. Pathological features were observed after hematoxylin-eosin staining, the distribution and content of Ki-67 in tumor were tested by IHC and the content of IL-12, IFN-γ, IL-4 and IL-10 in serum was determined by ELISA. Results:The inhibitory rate of Qingdu granules at low, middle and high dose and saikosaponin a was 30. 93%,43. 84% and 44. 17% and 43. 48%, respectively. The expression of Ki-67 was reduced in Qingdu granules groups and saikosaponin a group, the content of IL-12 and IFN-γ in serum was increased and the level of IL-4 and IL-10 was reduced in the above groups. Conclusion:Qingdu granules can inhibit breast cancer obviously, and the mechanism is probably related to the ability of immune system adjustment, which can enhance the antitumor effect.