Tissue engeneered bone in the shape of human TMJ condyle with marrow stem cells and porous natural coral / 实用口腔医学杂志

ABSTRACT

Objective: To prepare tissue engeneered bone in the shape of human TMJ condyle. Methods: Rabbit marrow stem cells (MSCs) were in vitro cultured and induced by rhBMP2.107 Cells were seeded into each piece of natural porous coral (NC) in the shape and in the size of 4 -year-old-child mandibular condyle. After two days in vitro incubation, six cell-coral complexes were implanted subcautanrously into the back of nude mice. Two months after operation, bone formation was observed by gross inspection,X-ray examination,scanning electronic microscope observation and histological observation. Results: New bone grafts in the shape of human mandibular condyle were successfully restored two months after implantation in all the samples. X-ray examination showed large amount of X-ray blocking shadow. NC was partially absorbed. New bone formation could be observed by electronic microscope observation and hostological observation on the surface and in the pores of NC. Conclusion: It is an effective method to fabricate bone graft in specific shape by seeding osteogenesis cells into natural coral in the wanted shape.