In vitro antimicrobial effect of polymethylmethacrylate denture base with silver-containing antimicrobial agents of nanometer level / 实用口腔医学杂志

ABSTRACT

Objective:To study the antimicrobial effect of polymethylmethacrylate (PMMA) denture base with silver-containing antimicrobial agents of nanometer level against Streptococcus mutans and Candida albicans in vitro. Methods:Minimum inhibitory concentration (MIC) of the silver-containing antimicrobial agent against S. mutans and C. albicans was examined. According to the MIC, 4 concentrations(1,2,5 and 10 mg/ml) of the agent were selected for the preparation of antimicrobial PMMA resin base. Then, the antimicrobial effect of the resin base was examined by in vitro bacteria culture and the most probable number(MPN) counting. Results:The MICs of the agent against S. mutans and C. albicans were 0.1 mg/ml and 1 mg/ml respectively. With the agent at 1, 2, 5 and 10 mg/ml, the inhibition ratios of the base against S.mutans were 67.4%,71.3%,99.0 and 99.5% respectively, that against C. albicans were 25.8%,54.8%,90.3% and 93.0%, respectively. Conclusion:The polymethylmethacrylate resin base with silver-containing antimicrobial agent of nanometer level at 5 mg/ml has ideal antimicrobial activity against S. mutans and C. albicans.