Effect of static magnetic field on the proliferation and differentiation of rat osteoblasts / 实用口腔医学杂志

ABSTRACT

Objective:To Study the effect of static magnetic field on the proliferation and differentiation of osteoblasts.Methods:In vitro cultured rat calvarial osteoblasts were seeded into 96-well culture plate and exposed to the different magnetic intensity field for 24,48 and 72 h respectively.MTT assay was applied to study the cell proliferation. ALP was measured by a microplate reader.Results:When the magnetic exposure time extended to 48 h or 72 h, the corresponding MTT value of osteoblasts with magnetic treatment of 40 mT or 62 mT increased obviously(P0.05). ALP activity increased after 24 h exposure to static magnetic field, the effect of static magnetic field intensity of 62 mT and 83 mT showed the strongest promotion effect(P