Detection of viral nucleic acid by biosensor
Journal of Preventive Medicine
; : 57-63, 2007.
Artigo
em Vietnamês
| WPRIM (Pacífico Ocidental)
| ID: wpr-671
Biblioteca responsável:
WPRO
ABSTRACT
Background:
DNA biosensor is based on micro-nano technologies aimed at developing a rapid diagnostic device of infectious diseases and diseases related to genetic change. Biosensors are compact size, high sensitivity and low cost\r\n', u'Objectives:
To evaluate effect of biosensor in detecting specific gene fragments of Herpes Simplex virus type 1 and 2 (HSV)\r\n', u'Subjects andmethod:
The electrical signals were recognized by means of transducer and from electrochemical detection of the hybridization between the probe 5\ufffd?AT CAC CGA CCC GGA GAG GGA C-3\ufffd?which were covalently immobilised onto the surface of micro electrodessensors in 3-aminopropyltri-ethoxysilance (APTS)-the conducting polymer matrix and the target (specific DNA sequences of HSV in the sample.\r\n', u'Results:
The DNA sensor offers a very high sensitivity, a fast response time, less than 1 min with the DNA target concentration up to 1nM in aqueous media at room temperature.However, in order to detect target DNA in the real samples, samples must be extracted DNA, denatured DNA sequences from a double fiber to single fiber. The measurement should be done soon\r\n', u'Conclusion:
The results show a large promise to develop quickly DNA sensors for widely application in bio-medical research \r\n', u'\r\n', u'
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Técnicas Biossensoriais
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Vietnamês
Revista:
Journal of Preventive Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo