Comparison of p16 status in primary hepatocellular carcinoma and its metastasis / 中国普通外科杂志

ABSTRACT

Objective To compare the p16 dynamic status in primary hepatocellular carcinoma(HCC) and its metastatic lesions.Methods The exon 2 of p16 gene was examined by PCR SSCP analysis in 36 paraffin embedded samples in 18 primary HCC cases and its metastatic lessions. Results p16 alterations were found in 4 (22.2%) of the 18 patients . Among them, 2 patients had alterations only in the metastatic lesions (one was deletion;the other was mutation).The other 2 patients had alterations both in primary and metastatic loci (one was deletion;the other was mutation).The deletion rate of exon 2 was 11.1%(2/18) and the mutation rate was 11.1%(2/18). However,the four alterations of p16 gene were found in 7 patients whose metastasis was accompanied by HCC recurrence ( 57.1% ), which was significantly higher than that in patients with metastasis but without HCC recurrence (P