Expression and purification of Helicobacter pylori urease A subunit and catalase / 中国免疫学杂志
Chinese Journal of Immunology
; (12)2001.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-674859
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
Investigated the effect of recombinant urease A subunit and catalase vaccines on protection against Helicobacter pylori (HP) infectionMethods:
Recombinant plasmids expressing UreA or KatA were constructed Expression was induced with IPTG,analyzed by SDS PAGE and Western blot UreA and KatA were purified with Bulk GST purification moduleResults:
The recombinant plasmids could express GST UreA and GST KatA fusion proteins,which accounted for 35% and 19% of total bacteria proteins respectively,and could specially react with antibody against GST The purity of the purified UreA and KatA was over 95%Conclusion:
The work laid a foundation for further studies of HP vaccine The recombinant vaccines may play an important role in preventing and treating HP infection and related diseases
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2001
Tipo de documento:
Artigo