Short-term Efficacy of Topical Immunosuppressive Agents on the Survival of Cultivated Allo-Conjunctival Equivalents
Korean Journal of Ophthalmology
; : 123-129, 2008.
Article
em En
| WPRIM
| ID: wpr-67682
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: To investigate the short-term efficacy of topical immunosuppressive agents on the survival of cultivated allo-conjunctival equivalents. METHODS: Twenty-five eyes of New Zealand white rabbits were included. Temporal conjunctivae were trephined to a diameter of 7.5 mm, and then cultured allo-conjunctival epithelial cells on amniotic membrane were transplanted onto them. Various immunosuppressants including steroid, cyclosporine, and rapamycin were applied topically four times a day for a week. Epithelial defects and graft edema were graded daily. Numbers of inflammatory cells were measured in H&E. PKH26 and cytokeratin 4 and 7 were immunostained. RESULTS: Earlier epithelialization was observed in 1% steroid-treated eyes and defects persisted significantly in 0.5% CsA applied eyes. In histology, PKH26 positive cells considered as donor cells were only found in 1% steroid or 0.01% rapamycin applied eyes. 1% steroid- or 0.01% rapamycin-applied eyes both showed positive staining for keratin-4 and -7. Inflammatory cells were less found in 1% steroid or 0.01% rapamycin treated eyes. CONCLUSIONS: Topical steroid or rapamycin can help to suppress acute inflammation and enhance the acute survival of transplanted conjunctival cells.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Compostos Orgânicos
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Transplante Homólogo
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Prednisona
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Contagem de Células
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Células Cultivadas
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Administração Tópica
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Ciclosporina
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Transplante de Células
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Túnica Conjuntiva
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Técnica Indireta de Fluorescência para Anticorpo
Limite:
Animals
Idioma:
En
Revista:
Korean Journal of Ophthalmology
Ano de publicação:
2008
Tipo de documento:
Article