Experimental studies on methionine dependence of human primary gastric cancer cells / 肠外与肠内营养
Parenteral & Enteral Nutrition
; (6)1997.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-677340
Biblioteca responsável:
WPRO
ABSTRACT
Objectives:
To study the methionine dependence (Met-dependence) of human primary gastric cancer cells in vitro when Met in the culture medicine was replaced by its precussor homocysteine(Hcy).Methods:
Fresh and sterile gaotric cancer samples and normal gastric mucosa were managed into single-cell suspension and were cultured in the Met -Hcy + and Met\++ Hcy\+- medium separately.proliferation of these cells in the two different culture media was examined by cell counter and flow cytometry (FCM).Results:
In the Met\++Hcy\+- medium,the growth of primary gastric cancer cells was inhibited,manifesting that the percentage of G 0G 1 tumor cells decreased and that of the S phase increased signiflcantly.However,normal gastric mucosal cells grew well and their cell cycles had no change.Conclusions:
Human primary gastric cancer cells are Met dependent in vitro whereas normal gastric mucosal cells have no similar results.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Parenteral & Enteral Nutrition
Ano de publicação:
1997
Tipo de documento:
Artigo