The effect of lipopoly-saccharide on glutamine metabolism in the rat Kupffer cells / 肠外与肠内营养

ABSTRACT

Objectives:To observe the metabolism of glutamine in the rat Kupffer cells and the effect of endotoxin. Methods:The changes of glutamine concentrations were measured before and 24 h after the in vitro cultures of the rat Kupffer cells with or without the stimulations of lipopoly saccharide. Results:Consumption and utilization rate of Gln in Kupffer cells with no LPS were (225?24)?mol/L and (9 33?0 99)% repectively.When the concention of LPS was 10.00 mg/L,consumptoin and utilization of Gln were (4 937?289)?mol/L and (73.29? 4.72 )%. Conclusions:Glutamine is an important substrate of Kupffer cell metabolism in the rest and LPS activated conditions.