Antigen presenting action and MHC limitation of Kupffer cells in immune response of halothane hepatitis / 第二军医大学学报

ABSTRACT

Objective:To study the antigen presenting of Kupffer cells in immune response of Guinea pig halothane hepatitis. Methods:Sixteen male Guinea pigs were randomly divided into 2 groups. Experimental group1% halothane and 40% O 2 were inhaled for 4 h, re inhaled on the 42nd and 84th day; Control grouponly inhaled 40% O 2. Lymphocytes and Kupffer cells were separated and the culture was mixed on the 21st day after the last inhalation. 3H TdR was added 18 h before the end of culture. Immune response and the antigen presenting action of Kupffer cells were analysis by lymphocyte transformation test (LTT). Results:Halothane had significant pro proliferative effects on autologous lymphocytes( P