Possible mechanism of mitochondria on anti-apoptotic effect of ginsenoside Rg1 on MPP~+-induced cellular apoptosis / 中国临床药理学与治疗学

ABSTRACT

AIM: To explore the possible anti apoptotic mechanism of ginsenoside Rg1 on MPP + induced cellular apoptosis. METHODS: The apoptosis of SHSY5Y induced by MPP + was observed by AO EB staining. Flow cytometry was used to quantitate mitochondrial transmembrane potential (△?m) and reactive oxygen species (ROS) . Western Blot was used to detect the expression of bcl 2 and bax proteins in SHSY5Y cells. RESULTS: MPP + induced apoptosis in SHSY5Y cells was obviously inhibited by pretreatment with 10 ?mol?L -1 Rg1. Although there was no difference of mitochondrial transmembrane potential between Rg1 pretreated groups and MPP + groups, the level of ROS in Rg1 pretreated groups decreased, the expression of bcl 2 protein increased and expression of bax protein decreased. CONCLUSION: Rg1 protects against MPP + induced apoptosis in SHSY5Y cells and the effect may be attributed to its remove of ROS and its regulation of expression of bcl 2 and bax proteins.