Determination of loureirin A and B in home-made resina draconis by RP-high-pressure liquid chromato- graphy / 第二军医大学学报

ABSTRACT

Objective: To determine loureirin A and B in different brands of home made resina draconis,providing basis for new quality control method.Methods: A high pressure liquid chromatography(HPLC) method has been developed. UV detector wavelength was set at 270 nm.The mobile phase was acetonitrile acetic acid solution (40∶60).The flow rate was 1.0 ml/min and the column was at room temperature.Results: A good linear correlation was found in the range of 4 to 24 ?g/ml of loureirin A.The regression equation obtained was Y =855.8+803 7.1 X ( r =0.999 9); A good linear correlation was found in the range of 20 to 120 ?g/ml of loureirin B.The regression equation obtained was Y =219.3+808 1.8 X ( r =0.999 9).Conclusion: The quantities of loureirin B in all brands are lower than the limit of quality standard.The quantities of loureirin A are higher than that of loureirin B in the same sample.