Effects of protein tyrosine kinase inhibitor on cerebrovascular smooth muscle cells Ca~(2+)store-operated Ca~(2+)influx / 中国药理学通报
Chinese Pharmacological Bulletin
; (12)1987.
Article
em Zh
| WPRIM
| ID: wpr-678057
Biblioteca responsável:
WPRO
ABSTRACT
AIM To study the effects of protein tyrosine kinase inhibitor and protein tyrosine phosphatase inhibitor on cultured bovine cerebrovascular smooth muscle cells (CSMC) Ca 2+ store operated Ca 2+ influx. METHODS Cell culture and single intracellular free Ca 2+ concentration was measured in fura 2/Am flueorescence probe by MetaFluor Fluorescence ratio imaging system. RESULTS (1) protein tyrosine kinase inhibitor (genistein,2.5,5,10 ?mol?L -1 )decreased Ca 2+ influx significantly induced by endothelin 1(ET 1),ATP,cyclopiazonic acid(CPA) in concentration dependent manner; (2) Protein tyrosine phosphatase inhibitor (vanadate,2,4,8 ?mol?L -1 ) increased Ca 2+ influx significantly induced by ET 1,ATP,CPA in concentration dependent manner. CONCLUSION Protein tyrosine kinase inhibitor and protein tyrosine phosphatase inhibitor have effects on Ca 2+ store operated Ca 2+ influx induced by ET 1, ATP, CPA. Protein tyrosine phosphorylation participats in the signal transduction of Ca 2+ store operat Ca 2+ influx in cerebrovascular smooth muscle cells.
Texto completo:
1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Pharmacological Bulletin
Ano de publicação:
1987
Tipo de documento:
Article