Regulation of the extracellular matrix synthesis and degradation in liver fibrotic rats by antisense TIMP_1 / 第三军医大学学报
Journal of Third Military Medical University
; (24)2003.
Article
em Zh
| WPRIM
| ID: wpr-678405
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WPRO
ABSTRACT
Objective To investigate the effects of antisense TIMP 1 on the interstitial collagen synthesis of fibrotic liver in rats. Methods Fibrosis was induced in the liver of rats with the injection of 60% CCl 4 and 5% alcohol. Recombinant antisense TIMP 1 gene mammalian expression vectors were construct and infused into the fibrotic liver of rats. The expressions of TIMP 1 mRNA, ?1(Ⅲ) mRNA and protein were determined by RT PCR, ISH and immunohistochemistry. The serum levels of PCⅠ, PCⅢ in rats were determined by RIA. Results Antisense TIMP 1 gene could markedly suppress the expression of endogenous TIMP 1 mRNA in the transfected liver of rats( P
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WPRIM
Idioma:
Zh
Revista:
Journal of Third Military Medical University
Ano de publicação:
2003
Tipo de documento:
Article