Influence of Astragalus membranceus and mung bean on arsenolite-induced rats / 中国临床药理学与治疗学

ABSTRACT

AIM: To investigate the effects of oral administration of Astragalus membranceus, mung bean and arsenolite on the toxic of the arsenolite induced rats and the possible mechanisms with metallothionein (MT). METHODS: All the rats were oral administration with arsenolite. The Astragalus membranceus and mung bean were compared with the cadmium chloride which induced MT synthesis. The contents of MT were determined by cadmium saturation method, the liver mRNA levels for MT 1, MT 2 were detected by RT PCR. The protective effects of renal and liver were observed by testing alanine aminotransferase (ALT), blood urea nitrogen (BUN) and serum creatinine (SCR). RESULTS: The contents of MT were accorded with the mRNA expression of MT 1, MT 2. Arsenolite, Astragalus membranceus and mung bean could induce the synthesis of MT, but the contents of MT which arsenolite induced were trace. The contents of MT significantly increased after oral administration of Astragalus membranceus and mung bean, especially in the Astragalus membranceus group (P