Determination of ephedrine alkaloids in Ephedra sinica by nonaqueous capillary electrophoresis / 中草药
Chinese Traditional and Herbal Drugs
; (24)1994.
Article
em Zh
| WPRIM
| ID: wpr-682279
Biblioteca responsável:
WPRO
ABSTRACT
Object To develop a rapid method for the determination of ephedrine alkaloids in Ephedra sinica Stapf by nonaqueous capillary electrophoresis (NACE) and evaluate the extracting method by determining the amount of alkaloids Methods The buffer contained 50 mmol/L ammonium acetate in methanol without any additives was used And the detection wavelength was 210 nm Results The best separation result was achieved within 8 min Linearity was obtained in range of 9 8-147 0 ?g/mL pseudoephedrine, 6 8-102 0 ?g/mL for norephedrin, 9 4-141 0 ?g/mL for ephedrine, 4 8-72 0 ?g/mL for norpseudoephdrine, 6 8-102 0 ?g/mL for methylephedrine respectively The recovery range of these five alkaloids was 95 0%-100 4% Conclusion This method is rapid and accurate for the quantitative analysis of ephedrin alkaloids in E sinica
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
1994
Tipo de documento:
Article