Effects of extracellular signal-regulated kinase on cell cycle and functions of rat cultured hepatic stellate cells stimulated by acetaldehyde / 中华消化杂志

ABSTRACT

Objective To investigate the effects of PD98059, the specific blocking agent of mitogen extracellular signal responsive kinase, on the cell cycle, cell proliferation, type Ⅰ collagen's secretion and transforming growth factor(TGF)? 1mRNA expression of rat hepatic stellate cells (HSC) stimulated by acetaldehyde. Methods Rat HSC stimulated by acetaldehyde were incubated with different concentration of PD98059. Cell cycle was analysed by flow cytometry. Cell proliferation was assessed by MTT colorimetric assay. Type Ⅰcollagen of culture medium was detected by enzyme linked immunoadsordent assay (ELISA). TGF? 1mRNA expression were examined by RT PCR. Results 20,50,100 ?mol/L PD98059 could significantly inhibit proliferation and induced G0/G1 phase arrest of HSC stimulated by acetaldehyde in a does dependent manner. 50,100 ?mol/L PD98059 could markedly inhibit type Ⅰcollagen's secretion and TGF? 1mRNA expression of HSC stimulated by acetaldehyde. Conclusion The extracellular signal regulated kinase signal transduction passway regulates the cell proliferation, type Ⅰcollagen's secretion and TGF? 1mRNA expression of rat HSC stimulated by acetaldehyde,which might be partly related to its regulative effect on the cell cycle.