SELECTION FOR URA5 MUTANTS OF CRYPTOCCOCUS NEOFORMANS CAPSULE-DEFICIENT STRAIN CAP59 / 微生物学通报

ABSTRACT

This study was to improve the way for selecting ura5 mutants of Cryptoccocus neoformans Cap59 capsule-deficient strains.They were induced by Diethyl Sulfate. Ura5 mutants were screened by 5-fluoroorotic acid counter selection method. Using the new method, we obtained two ura5 mutants of Cryptoccocus neoformans Cap59 capsule-deficient strain.A easy method that was used to screen ura5 mutants of Cryptoccocus neoformans has been established.