Effects of culture medium osmolarity on the proliferation of human hepatoma cells / 医学研究生学报
Journal of Medical Postgraduates
; (12)2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-684541
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To investigate the effects of culture medium osmolarity on the proliferation of human hepatoma cell line HHCC.Methods:
Cell proliferation was analyzed by MTT assay and cell cycle analysis was carried out by flow cytometry.Results:
Hyperosmolarity decreased significantly the absorbance (A) of HHCC cells and cell number, and hyposmolarity have counter effects. Compared with control, hyperosmolarity blocked cell cycle progression at the G 1 phase, while hyposmolarity accelerated cells into S phase from G 1 phase, and the effect of hyposmolarity was weakened by chloride channel blocker NFA.Conclusion:
Culture medium osmolarity influenced the proliferation of HHCC cells by mediating cell cycle.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Medical Postgraduates
Ano de publicação:
2003
Tipo de documento:
Artigo