Selection of bFGF Mimic Peptide by Phage Display / 中国生物工程杂志
China Biotechnology
; (12)2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-684956
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To obtain the bFGF mimic peptide binding to FGFR via phage display, and to provide the base for developing peptide agonist of bFGF.Methods:
Using Balb/c 3T3 cells as the target cells and COS-7 cells as the subtractive panning, the phage display heptapeptide library was biopanned for 4 rounds to obtain the single phage clones. The affinity and the specificity of the clones were assessed by ELISA. DNA sequencing was applied to further analyze the positive clones.Results:
Twelve positive clones were selected from the enriched phages. A group of hydrophobic peptides containing a conserved motif, PR, was identified.Conclusion:
Two bFGF mimic heptapeptides binding to FGFR were selected, which may be used as the candidates for bFGF agonist.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
China Biotechnology
Ano de publicação:
2006
Tipo de documento:
Artigo