Inhibitory effect of TNF-?on expression of hTERT and mdr1 gene in leukemic K562 and K562/ADM cell lines / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
; (6)2006.
Article
em Zh
| WPRIM
| ID: wpr-685641
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WPRO
ABSTRACT
Objective:To investigate the inhibitory effect of TNF-?on hTERT gene expression in human myelogenous leukemia cell line K562 and K562/ADM and to study the influence of changed telomerase activity on expression of multi- drug resistance-1(mdr 1)gene.Methods:K562 and K562/ADM cells were treated with 5?10~6 U/L TNF-?for 24 h, then cell proliferation was detected by MTT assay and cell apoptosis was detected by flow cytometry.The expression of hTERT and mdrl mRNA was detected by RT-PCR and the telomerase activity was detected by ELISA.Results:TNF-?in- hibited the growth of K562 and K562/ADM cells and the inhibition showed a time-effect relationship(P
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Zh
Revista:
Chinese Journal of Cancer Biotherapy
Ano de publicação:
2006
Tipo de documento:
Article