Mass Culture and Purification of Vero-based Influenza Virus / 中国生物工程杂志
China Biotechnology
; (12)2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-686096
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
Establish mass-scale purification technology of cell-derived influenza virus.Methods:
A microcarrier-based process was used to produce human influenza virus in serum free-adapted Vero cells. The virus was purified in a sequence of downstream processing steps including inactivation, clarification, anion exchange chromatography, affinity chromatography and size-exclusion chromatography.Results:
The recovery of HA reached 102%. 95.3% total protein, including 99.77% host cell protein, and 99.99% host cell DNA were removed during downstream processing.Conclusion:
Providing a high effective purification technology for cell-derived influenza virus.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Biotechnology
Ano de publicação:
2006
Tipo de documento:
Artigo