Vancomycin-based fluorescent enzyme-linked immunoabsorbent assay for detection of Staphylococcus aureus / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1510-1517, 2018.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-687668
Biblioteca responsável:
WPRO
ABSTRACT
In the study, fluorescent enzyme-linked immnoabsorbent assay for detection of Staphylococcus aureus was established with IgG from pig as capture antibody and quantum dot nanobeads (QBs) labeled vancomycin (QB-Vans) as testing antibody. Quantum dot of about 100 nm partical size nanobeads were prepared and linked with vancomycin. The optimum concentrations of salt ions were 0.01 mol/L, and the optimum pH was 6.0. Under the optimum conditions, the detection sensitivity for S. aureus was 10⁴ CFU/mL, and there was no cross-reaction with other pathogenic bacteria. Thus, the method could be used for rapid screening of S. aureus, for the clinical monitoring and foodborne pathogens detection.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2018
Tipo de documento:
Artigo