Role of MBP-1 in proliferation, apoptosis and invasion of human esophageal cancer cells Ec109 / 重庆医学

ABSTRACT

Objective To investigate the role of MBP-1 in proliferation,apoptosis and invasion of human esophageal cancer cells Ec109.Methods The human esophageal cancer cells Ec109 were cultured,and divided into the MBP-1 mimics group,siRNA-MBP-1 group,negative control group and blank control group.The cell proliferation activity of each group was detected by tetrazolium blue (MTT) method;flow cytometry was used to detect cell apoptosis and cell cycle;Transwell assay was used to detected the invasion ability and the expressions of cellular cycle related C-myc,Cyclin D1 and Cyclin E were detected by western blot.Results Compared with the negative control group and blank control group,the expression of MBP-1 in esophageal cancer cells Ec109 of the MBP-1 mimics group was up-regulated (P＜0.05),the proliferation ability of esophageal cancer cells was decreased,increased the proportion of apoptosis,decreased the proportion of G0/G1 phase cells,inhibited the number of invasive cells was decreased and the expressions of C-myc,Cyclin D1 and Cyclin E proteins.After silencing MBP-1,the expression of MBP-1 in esophageal cancer cells Ec109 in the siRNA-MBP-1 group was down-regulated,the proliferation ability of esophageal cancer cells was increased,the proportion of apoptosis was decreased,the proportion of G0/G1 phase cells was increased.the number of invasive cells was increased and the expressions of C-myc,Cyclin D1 and Cyclin E proteins were up-regulated.Conclusion MBP-1 is closely correlated with the cell proliferation,cell apoptosis and invasion ability of human esophageal cancer cell line Ec109,and its mechanism might be related to cell cycle abnormality.