Comparison of differences of Notch1 gene methylation between hyperplastic lesions tissues and breast cancer tissues in Uyghur women / 临床与实验病理学杂志

ABSTRACT

Purpose To compare the difference of Notch1 methylation in the breast cancer and hyperplastic lesions tissue from Uyghur in Xinjiang. Methods The methylation level of Notch1 gene in Uyghur breast tissues including usual ductal hyperplasia (UDH), atypical ductal hyperplasia (ADH), ductal carcinoma in situ (DCIS), and invasive ductal carcinoma(IDC) were detected by MALDI-TOF-MS technique. The association of the methylation level with clinical pathological characteristics of patients was analyzed. The expression of Notch 1 protein was detected by immunohistochemistry. The relationship between the methylation status and expression was assay. Results The methylation rate of Notch l gene in UDH, ADH, DCIS, and IDC group was gradually decreased (P＜0.05). The 9 CpG sites methylation level of 13 CpG sites are statistically lower in cancer tissues (P＜0.05). The hypomethylation are accompanied with low differentiation, lymph node metastasis and high stage of TNM (P＜0.05). The lower DNA methylation is negatively correlated with the expression of Notch l (P＜0.05). Conclusion There was a differences of the expression and methylation rate of Notch 1 between breast cancer and hyperplastic lesions tissue, and its biological significances needs to be further studied.