Study on detection of mouse influenza serum by surface enhanced raman spectroscopy / 实用医学杂志

ABSTRACT

Objective A new method for serum detection based on surface-enhanced Raman spectroscopy was explored by comparing the Raman spectra between normal mice and influenza virus-infected mice. Methods The nano-silver sol was used as the active substrate.The Raman spectra of the normal group,the model group and the Tamiflu control group were detected by portable Raman spectroscopy,and the partial least squares discrimina-tion analysis(OPLS-DA)was performed by the orthogonal correction. The number of RNA replicas of influenza virus in lung tissue was measured by RT-PCR as a control method. Results At the 3rd or 5th days,the serum of the normal group,the model group and the Tamiflu control group showed a significant trend. By the ROC curve evaluation,the predictive ability of 3 groups of serum SERS models established by OPLS-DA was high,which could distinguish and differentiate 3 groups of serum. Conclusions The results of SERS and RT-PCR detections were consistent.The preliminary results show that SERS pattern can help to identify and diagnose whether the body is infected with influenza virus.