Renal protective and anti-apoptotic functions of erythropoietin in ze-brafish embryonic development / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12): 1067-1074, 2018.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-701241
Biblioteca responsável:
WPRO
ABSTRACT
AIM:
To analyze zebrafish embryos and to identify erythropoietin (Epo) as an active renal anti-apoptotic factor in an Epo receptor (EpoR)-dependent manner.METHODS:
For transient knockdown of Epo and EpoR in renal Tg (wt1bEGFP) zebrafish reporter lines, the morpholino antisense oligonucleotide technique was used. Morphant zebrafish embryos were phenotypically analyzed using fluorescence microscopy. Apoptosis was determined by TUNEL assay and Annexin V staining. Western blot was used to identify Akt phosphorylation in Epo and EpoR knockdown zebrafish. RE-SULTSEpo and EpoR zebrafish morphants exhibited pathophysiological changes within the pronephros, adversely affecting pronephric structure. Zebrafish embryos upon silencing of Epoa and EpoR showed a significant increase in the apoptosis within the zebrafish pronephros, consequently leading to renal pathophysiological effects. Decreased p-Akt was identified in Epo and EpoR knockdown zebrafish embryos.CONCLUSION:
Epo is an essential regulator of renal development and function by interacting with its receptor EpoR and thereby repressing apoptosis, mechanistically by promoting Akt phospho-rylation.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
2018
Tipo de documento:
Artigo