Effect of Bifidobacterium combined with ulinastatin on immunologic function of CLP rats / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effect of Bifidobacterium combined with ulinastatin on the immunologic function of sepsis rats.Methods:One hundred male SD rats were randomly divided into sham-operated group,model group,Bifidobacterium treatment group,Ulinastatin treatment group,combining treatment group.Cecal ligation and punctured was used to prepare sepsis model in rats.Survival rate was observed.The number of intestinal floras were determined.The tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6)were tested by enzyme linked immunosorbent assay (ELISA).T-lymphocyte subset was tested by flow cytometry.Serum endotoxin was tested by TAL method.Results:Serum endotoxin,Escherichia coli,CD8+T cell,TNF-α and IL-1β of model group were significantly higher than the sham operation group (P＜0.05).CD3+,CD4+T cell and ratio of CD4+/CD8+T cell,Bifidobacterium and lactobacillus were significantly higher than the sham operation group (P＜0.05).Compared with model group,Serum endotoxin,Escherichia coli,CD8 +T cell,TNF-α and IL-1 β of combining treatment group were significantly decrease (P＜0.05).CD3+,CD4+T cell and ratio of C D4+/CD8+T cell,Bifidobacterium and lactobacillus were significantly increase (P＜0.05).Serum endotoxin,TNF-α and IL-1 β of combining treatment group were significantly lower than that in Bifidobacterium treatment group and Ulinastatin treatment group.CD3+,CD4+T cell and ratio of CD4+/CD8+T cell,Bifidobacterium and lactobacillus were significantly higher than that in Bifidobacterium treatment group and Ulinastatin treatment group.Conclusion:Efficacy is significantly improvement while Bifidobacterium combined with ulinastatin,which can restrain TNF-α and IL-6 of CLP rats,decrease the increasing of serum endotoxin,and regulate the balance of intestinal floras,also improve the immune function of CLP rats.