Inhibitory effects of tamoxifen on thyroid papillary carcinoma / 中国现代普通外科进展
Chinese Journal of Current Advances in General Surgery
; (4): 856-860, 2017.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-703770
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To explore the effects of tamoxifen on proliferation,migration and invasion of human thyroid papillary carcinoma BCPAP cells.Methods:
Human thyroid papillary carcinoma BCPAP cells were treated with various concentrations of tamoxifen for 24 hours,respectively,CCK8 assay was used to detect the change of proliferation level;It can be divided into four groupsnegative group,10 μmol/L TAM group and 20 μmol/L TAM group.Wound healing test was used to detect the change of migration ability and Transwell assay was used to detect the change of invasion ability.The protein levels of bcl-2 and C-myc in the BCPAP cells treated with various concentrations of tamoxifen were detected by Western blot.Results:
CCK8 assay,wound healing test and Transwell assay showed that tamoxifen had induced a dose-dependent decrease in the proliferation,migration and invasion of human thyroid papillary carcinoma BCPAP cells.Furthermore,western blot demonstrated that the tamoxifen decreased the expression of bcl-2 and C-myc in BAPAP cells.Conclusions Tamoxifen can inhibit proliferation,migration and invasion of human thyroid papillary carcinoma BCPAP cells.We speculated that the inhibition of proliferation maybe related to down-regulation of bcl-2 and C-myc expression.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Current Advances in General Surgery
Ano de publicação:
2017
Tipo de documento:
Artigo