Preparation of Rotundic Acid Monomer and Content Determination of 3 Components in Ilicis rotundae and Its Refined Products by RP-HPLC / 中国药房
China Pharmacy
; (12): 326-329, 2018.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-704577
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:
To prepare high-purity rotundic acid, and to determine the contents of syringoside, peduncloside and rotundic acid in Ilicis rotundae and its refined products.METHODS:
I. rotundae saponins was prepared by ethanol extraction method and hydrolyzed with methanol and sodium hydroxide; rotundic acid monomer was obtained after recrystallization. The contents of syringoside, peduncloside and rotundic acid in I. rotundae, its refined product 1 and its refined product 2 were determined by RP-HPLC. The determination was performed on an Agilent XDB C18 column with mobile phase consisted of water-acetonitrile (gradient elution) at the flow rate of 1. 2 mL/min. The column temperature was 30 ℃, and the detection wavelength was set at 210 nm. The sample size was 10 μL.RESULTS:
The purity of rotundic acid exceeded 99%. The linear ranges of syringing, peduncloside and rotundic acid were 0. 18-3. 22, 0. 59-10. 36 and 0. 20-3. 53 μg, respectively (r=0. 999 9). RSDs of precision, stability (24 h) and reproducibility tests were all lower than 2. 0% (n=6-7). The average recoveries were 96. 08%-100. 81 % (RSD≤1. 98%, n=6). The contents of syringoside,peduncloside and rotundic acid in I. rotundae were 1. 61%,7. 26%, 1. 29% (RSD≤1. 08%,n=3); those of refined product 1 were 0,82. 59%,4. 18% (RSD≤1. 67%,n=3); those of refined product 2 were 0, 73. 29%, 7. 41% (RSD≤1. 15%,n=3),respectively.CONCLUSIONS:
Prepared rotundic acid has high purity and is simple in preparation, safe and environmentally-friendly. RP-HPLC method that established is simple and reliable, and can meet the requirements for rapid analysis of I. rotundae and its refined products.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2018
Tipo de documento:
Artigo