Effects of Xuesaitong on the Pharmacokinetics of Losartan in Rats Determined by HPLC-MS / 中国药师

ABSTRACT

Objective:To develop an HPLC-MS determination method for the effects of Xuesaitong on the pharmacokinetics of losartan in rats. Methods:After oral administration of single losartan and combined usage of losartan and Xusaitong in rats,the con-centrations of losartan and EXP3174 in plasma were determined by HPLC-MS. The main pharmacokinetic parameters were calculated and compared by statistical analysis. Results:The calibration curves of losartan and EXP3174 were linear within the range of 2.0-2 000 ng·ml-1(r=0.996 4 and r=0.999 5). The average recovery was 85.19%-109.79%. The results showed that after oral co-ad-ministration with Xuesaitong,t1/2and Vz/F of losartan significantly decreased (P<0.05), Tmaxsignificantly increased (P<0.05), t1/2of EXP3174 significantly decreased(P<0.05),and Tmaxsignificantly increased(P<0.05). Conclusion:The HPLC-MS method is sensitive and selective,and suitable for the detection of losartan and EXP3174 in plasma in vivo. The experiments showed that Xue-saitong can affect the absorption and metabolism of losartan in vivo.