Effects of Xuesaitong on the Pharmacokinetics of Losartan in Rats Determined by HPLC-MS / 中国药师
China Pharmacist
; (12): 1937-1941,1949, 2017.
Article
em Zh
| WPRIM
| ID: wpr-705394
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WPRO
ABSTRACT
Objective:To develop an HPLC-MS determination method for the effects of Xuesaitong on the pharmacokinetics of losartan in rats. Methods:After oral administration of single losartan and combined usage of losartan and Xusaitong in rats,the con-centrations of losartan and EXP3174 in plasma were determined by HPLC-MS. The main pharmacokinetic parameters were calculated and compared by statistical analysis. Results:The calibration curves of losartan and EXP3174 were linear within the range of 2.0-2 000 ng·ml-1(r=0.996 4 and r=0.999 5). The average recovery was 85.19%-109.79%. The results showed that after oral co-ad-ministration with Xuesaitong,t1/2and Vz/F of losartan significantly decreased (P<0.05), Tmaxsignificantly increased (P<0.05), t1/2of EXP3174 significantly decreased(P<0.05),and Tmaxsignificantly increased(P<0.05). Conclusion:The HPLC-MS method is sensitive and selective,and suitable for the detection of losartan and EXP3174 in plasma in vivo. The experiments showed that Xue-saitong can affect the absorption and metabolism of losartan in vivo.
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WPRIM
Idioma:
Zh
Revista:
China Pharmacist
Ano de publicação:
2017
Tipo de documento:
Article