Simultaneous determination of five constituents in Simotang Oral Liquid by HPLC / 中成药
Chinese Traditional Patent Medicine
; (12): 114-117, 2018.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-710165
Biblioteca responsável:
WPRO
ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of five constituents in Simotang Oral Liquid (Aucklandiae Radix,Aurantii Fructus,Arecae Semen,etc.).METHODS The analysis of 50% methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent Zorbax C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1% formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 283 nm.RESULTS Synephrine,norisoboldine,naringin,hesperidin and neohesperidin showed good linear relationships within the ranges of 5.8-185.6 μg/mL (r =0.999 9),0.829-26.52 μg/mL (r =1.000 0),9.775-312.8 μg/mL (r =1.000 0),0.594-19 μg/mL (r =0.999 5) and 5.2-166.4 μg/mL (r =1.000 0),whose average recoveries were 98.93%,98.95%,102.57%,99.67% and 103.43% with the RSDs of 1.85%,1.27%,0.52%,0.89% and 0.43%,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the rapid quality control of Simotang Oral Liquid.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional Patent Medicine
Ano de publicação:
2018
Tipo de documento:
Artigo